男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12細(xì)胞, 小鼠成肌細(xì)胞

C2C12細(xì)胞, 小鼠成肌細(xì)胞

簡(jiǎn)要描述:C2C12細(xì)胞, 小鼠成肌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:2047

產(chǎn)品分類

Product Category

詳細(xì)介紹

C2C12細(xì)胞, 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™    Price:$256.00
Designations:C2C12Biosafety Level:1Shipped:frozenMedium Properties:& Serum:oSee PropagationGrowthadherentOrganism:Mus musculus (muse)Morphology:myoblastSource:Strain: C3H Tissue: muscle Cell Type: myoblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. C2C12細(xì)胞, 小鼠成肌細(xì)胞Applications:transfection host (Nucleofection technology from LonzaRoche FuGENE® Transfection Reagents)Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested and found negative for ectromelia virus (mousepox).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.Incubate cultures at 37°C.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男女18禁啪啪无遮挡激烈网站,鸭子TV国产在线永久播放,最近中文字幕在线MV视频在线,四川妇女BBBB的创业精神

                  久久久麻豆视频| 91精品一区二区三区久久久久久 | 中国娇小与黑人巨大交| 无码国产色欲XXXX视频| 内射美女少妇| 91久久精品美女高潮| 日本妞被黑人狂躁高潮| 后入内射欧美99二区视频| 体验区试看120秒啪啪免费| 性少妇freesexvideos高清?| 91精品国产一区二区三区蜜臀| 中文字幕色哟哟| gogogo高清视频大全| 乱码久久| 成人h动漫精品一区二区下载| 国产一级av国产免费| 大地影视第二页在线观看高清版| 国产探花在线精品一区二区| 国产欧美精品区一区二区三区| 亚洲黄色国产| 日韩精品欧美一区| 国产精品欧美激情在线| 97自拍视频在线| 日本女人性视频| 久久亚洲国产精品尤物| 98精品一区二区三区| 亚洲AV无码国产精品永久一区| 国产精品一级黄色片| 泰国《肉欲》电影| 国产精品久久综合青草亚洲av| 国产精品久久久久高潮色老头| 无码人妻精品一区二区三区东京热| 亚洲中文字幕97久久精品少妇| 欧美日韩人妻| 超碰av在线观看| 高潮久久久久久| 久久国产成人精品av| 大吊一区二区三区| 国产亲子乱A片免费视频| 亚洲精品a人在线观看| 亚洲一级片在线观看|